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HIV infection in serodiscordant couples does not have impact on the embryo quality and ICSI outcome

HIV infection in serodiscordant couples does not have impact on the embryo quality and ICSI outcome

Authors: Marrco Melo, Marcos Meseguer, José Bellver, José Remohí, Antonio Pellicer, Nicolás Garrido

(Fertility and Sterility 2008; 89: 141-150)

OBJECTIVE: To evaluate the embryo quality in our program for HIV-1 serodiscordant couples (SDC) with the male infected in comparison with a tubal infertility control group.

DESIGN: Retrospective case-control study.

SETTING: Instituto Universitario IVI, Valencia, Spain PATIENTS: 30 SDC and 79 control couples without HIV-1 infection attending for ICSI. Only first cycles were considered.

INTERVENTION: Controlled ovarian hyperstimulation and ICSI, in both groups. In SDC group: sperm wash, nested polymerase chain reaction (PCR) in semen sample and capacitation by swim-up, after thawing semen sample. In control group: sperm capacitation by swim-up, after thawing semen sample.

MAIN OUTCOME MEASURE: ICSI procedure and embryo characteristics (fertilization, cleavage, embryo morphology and development) and cycle outcome (ongoing pregnancy and miscarriage rates).

RESULTS: Fertilization and cleavage rates were similar between the groups. On days 2 and 3 of embryo development, very similar embryo features were found between the groups. There was no difference in mean number of optimal embryos on day 3. When embryos were cultured up to 5- 6 days, a significant increase in embryo blockage was found in SDC group, in comparison with the control group. The mean number of optimal blastocysts on day 6 was comparable in both groups. No difference was found regarding number of cryopreserved and transferred embryos, implantation, pregnancy, multiple
pregnancy, and miscarriage rates between the groups.

CONCLUSIONS: HIV-1 infection in SDC with infected males does not appear to have a significantly negative impact on the embryo development and ICSI outcome.

KEYWORDS: embryo quality/ HIV-1/ ICSI/ nested PCR/ semen wash/ serodiscordant couples